http://teknokes.poltekkesdepkes-sby.ac.id/index.php/Teknokes/issue/feed 2023-04-13T17:06:03+00:00 Dr. Triwiyanto triwi@poltekkesdepkes-sby.ac.id Open Journal Systems <div style="text-align: justify;">The TEKNOKES is a peer-reviewed periodical scientific journal aimed at publishing research results of the Journal focus areas. <div style="text-align: justify;">The Journal is published by the Department of Electromedical Engineering, Health Polytechnic of Surabaya, Ministry of Health Indonesia. The role of the Journal is to facilitate contacts between research centers and the industry. The aspiration of the Editors is to publish high-quality scientific professional papers presenting works of significant scientific teams, experienced and well-established authors as well as postgraduate students and beginning researchers. All articles are subject to anonymous review processes by at least two independent expert reviewers prior to publishing on the Indonesian Journal of Electronics, Electromedical Engineering, and Medical Informatics website.</div> </div> http://teknokes.poltekkesdepkes-sby.ac.id/index.php/Teknokes/article/view/505 2023-04-13T17:06:03+00:00 Yunia Hidayati james.p.simanjuntak@poltekkesjambi.ac.id Asnaily Asnaily james.p.simanjuntak@poltekkesjambi.ac.id Akhirul Jumaisal james.p.simanjuntak@poltekkesjambi.ac.id James Perdinan Simanjuntak james.p.simanjuntak@poltekkesjambi.ac.id

<p><em>Candida albicans </em>is a commensal on the oral mucosal surfaces of healthy individuals but can be pathogenic in immunocompromised individuals. The germ tube test is an inexpensive, faster, and easier method used to identify and differentiate it from other yeast species. Generally, the test is carried out using serum as the medium. Plasma overall has the same characteristics as serum and differs only in the content of clotting factors. In addition, plasma is easier to obtain than serum in most healthcare services. This study aims to demonstrate three types of plasma as alternative media for germ tube test. CPDA (Citrate Phosphate Dextrose Adenine), EDTA (Ethylenediaminetetraacetic), and Sodium Citrate plasma were compared to serum in producing of germ tubes. Each plasma and serum was tested in five repetitions. The experimental method was carried out in Mycology Laboratory at Health Polytechnic of Jambi. The data were observed based on the germ tube formation time and analyzed with one-way ANOVA test. The result showed the lengths of germ tube formation time were significantly different. Although CPDA plasma was also significantly different compared to serum, it showed the average time which is at the minimum limit of the incubation time range that is recommended in the standard protocol of the test. This study concluded that plasma can be used as a substitute for serum in the germ tube tes, especially CPDA plasma, as it is faster in formating time, easier to obtain, and considered safer to use than others.</p>

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